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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
16/11/2022 |
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Data da última atualização: |
16/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASARIN, T.; FREITAS, N. C.; PINTO, R. T.; BREITLER, J. C.; RODRIGUES, L. A. Z.; MARRACCINI, P.; ETIENNE, H.; DINIZ, L. E. C.; ANDRADE, A. C.; PAIVA, L. V. |
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Afiliação: |
TATIANE CASARIN, UNIVERSIDADE FEDERAL DE LAVRAS; NATÁLIA CHAGAS FREITAS, UNIVERSIDADE FEDERAL DE LAVRAS; RENAN TERASSI PINTO, UNIVERSIDADE FEDERAL DE LAVRAS; JEAN‐CHRISTOPHE BREITLER, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEONARDO AUGUSTO ZEBRAL RODRIGUES, UNIVERSIDADE FEDERAL DE LAVRAS; PIERRE MARRACCINI, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; HERVÉ ETIENNE, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRÍCOLA PARA O DESENVOLVIMENTO; LEANDRO EUGENIO CARDAMONE DINIZ, CNPSO; ALAN CARVALHO ANDRADE, CNPCa; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 17270, 2022. 10 p. |
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DOI: |
https://doi.org/10.1038/s41598-022-21566-w |
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Idioma: |
Inglês |
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Conteúdo: |
Coffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus. MenosCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacte... Mostrar Tudo |
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Thesagro: |
Biodiversidade; Café Robusta; Coffea Canephora; Genótipo; Melhoramento Genético Vegetal; Mutação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148303/1/Multiplex-CRISPRCas9-mediated.pdf
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Marc: |
LEADER 02597naa a2200313 a 4500
001 2148303
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-21566-w$2DOI
100 1 $aCASARIN, T.
245 $aMultiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora.$h[electronic resource]
260 $c2022
520 $aCoffea canephora (2n=2x22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different exons of the CcPDS gene were used. The sgRNAs were under the C. canephora U6 promoter regulation. The target gene encodes phytoene desaturase, an enzyme essential for photosynthesis involved in B-carotene biosynthesis. Somatic seedlings and embryos with albino, variegated and green phenotypes regenerated after Agrobacterium tumefaciens-mediated genetic transformation were analyzed by verifying the insertion of the Cas9 gene and later by sequencing the sgRNAs target regions in the genome of Robusta modified seedlings. Among them, 77% had the expected mutations, and of which, 50% of them had at least one target with a homozygous mutation. The genotype, temperature of co-cultivation with the bacteria, and light intensity used for subsequent embryo regeneration appeared to strongly influence the successful regeneration of plants with a mutated CcPDS gene in the Coffea genus.
650 $aBiodiversidade
650 $aCafé Robusta
650 $aCoffea Canephora
650 $aGenótipo
650 $aMelhoramento Genético Vegetal
650 $aMutação
700 1 $aFREITAS, N. C.
700 1 $aPINTO, R. T.
700 1 $aBREITLER, J. C.
700 1 $aRODRIGUES, L. A. Z.
700 1 $aMARRACCINI, P.
700 1 $aETIENNE, H.
700 1 $aDINIZ, L. E. C.
700 1 $aANDRADE, A. C.
700 1 $aPAIVA, L. V.
773 $tScientific Reports$gv. 12, 17270, 2022. 10 p.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
27/01/2017 |
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Data da última atualização: |
27/01/2017 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
SOUSA, V. G. de; BRIENZA JUNIOR, S.; BARBOSA, M. G.; MARTORANO, L. G.; SILVA, V. C. |
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Afiliação: |
Vanessa Gomes de Sousa; SILVIO BRIENZA JUNIOR, CPATU; Maricélia Gonçalves Barbosa, Secretaria de Estado de Meio Ambiente e Sustentabilidade; LUCIETA GUERREIRO MARTORANO, CPATU; Verônica Chaves Silva, MESTRANDA UFPA. |
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Título: |
Taxi-branco (Tachigali vulgaris L.F. Gomes da Silva & H.C. Lima): botânica, ecologia e silvicultura. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Belém, PA: Embrapa Amazônia Oriental, 2016. |
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Páginas: |
37 p. |
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Série: |
(Embrapa Amazônia Oriental. Documentos, 426). |
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Idioma: |
Português |
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Conteúdo: |
Nesta publicação estão apresentadas as principais informações sobre a espécie que vem ganhando espaço em plantios florestais da região. |
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Palavras-Chave: |
Taxi-branco. |
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Thesagro: |
Botânica; Ecologia; Silvicultura. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154191/1/DOCUMENTOS-426-Ainfo.pdf
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Marc: |
LEADER 00814nam a2200229 a 4500
001 2062064
005 2017-01-27
008 2016 bl uuuu u0uu1 u #d
100 1 $aSOUSA, V. G. de
245 $aTaxi-branco (Tachigali vulgaris L.F. Gomes da Silva & H.C. Lima)$bbotânica, ecologia e silvicultura.
260 $aBelém, PA: Embrapa Amazônia Oriental$c2016
300 $a37 p.
490 $a(Embrapa Amazônia Oriental. Documentos, 426).
520 $aNesta publicação estão apresentadas as principais informações sobre a espécie que vem ganhando espaço em plantios florestais da região.
650 $aBotânica
650 $aEcologia
650 $aSilvicultura
653 $aTaxi-branco
700 1 $aBRIENZA JUNIOR, S.
700 1 $aBARBOSA, M. G.
700 1 $aMARTORANO, L. G.
700 1 $aSILVA, V. C.
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Embrapa Amazônia Oriental (CPATU) |
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